By Ben Jones
The visitors will make the short trip from Bedfont Sports Club, a venue familiar to the Beavers as they use it as their training ground.
The winners of the tie will move into the quarter-finals and will face an away match against National League Barnet.
The cups have been a source of success for Hampton having reached the FA Cup first round this season and progressed against Spelthorne Sports in the Charity Cup.
Hampton are five-time winners of this competition, with their last success coming in 2016-17 with victory over Wealdstone. Gary McCann lifted the trophy last season with Hendon courtesy of a penalty shootout win over Staines.
It is likely that several first team players will be rested as others are given minutes. Ogo Obi and Zak Joseph could be in line for recalls and Karl Oliyide could make his first start for the club. Simon Downer could be rested ahead of the visit of high-flying Welling United on Saturday in the league.
Hampton come into the match off the back of a 0-0 draw away to Gloucester City and will be looking to find their scoring boots this time around.
Bedfont progressed to this round after a 4-2 victory at home to Southall, courtesy of an Abdeen Temitope Abdul hat-trick.
On Saturday, they came back from 2-0 down to draw against play-off chasing Cheshunt in the Isthmian League South Central Division. This brought an end to a run of four consecutive wins.
After being promoted last season, they sit in 13th place and have scored 29 goals - six more than the league leaders - but have conceded 29.
The two sides met in the first pre-season friendly of the year with Hampton winning 7-2 and there certainly looks to be potential for another high scoring game.